Tretinoin nrf2 reporter gene assay
WebOverexpression of Nrf2 increased the activity of the nrf2 promoter-luciferase reporter, while expression of mutant Nrf2 protein repressed activity. Thus, Nrf2 appears to autoregulate … WebKeratinoSens TM is a cell-based reporter gene assay that models the second key event in the Adverse Outcome Pathway for Dermal Sensitization (keratinocyte activation). The …
Tretinoin nrf2 reporter gene assay
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WebSep 28, 2024 · Nuclear factor erythroid-2 related factor 2 (NRF2) encoded by the NFE2L2 gene is a transcription factor critical for protecting cells from chemically-induced oxidative stress. We developed computational procedures to identify chemical modulators of NRF2 in a large database of human microarray data. A gene expression biomarker was built from … WebAug 11, 2009 · (B) Reporter gene assay was performed as described above using NQO1-ARE-dependent luciferase reporter gene construct in MDA-MB-231 cells. (C) Total cell lysates from one of the reporter gene assays were subjected to immunoblot analysis with anti-HA antibodies for detection of Nrf2.
WebAssay Nrf2 in 3 hr 30 min in cell/tissue extracts with Nrf2 Transcription Factor Assay Kit ab207223. Sensitivity > 600 ng/well. Cited in > 26 publications. ... Nrf2 is released from INrf2, translocates to the nucleus, and results in the activation of ARE-mediated gene expression. Nrf2 is also synthesized de novo after exposure to stress. WebMar 24, 2024 · Nrf2 is an essential transcription factor regulating the expression of genes containing an electrophile-responsive element (EpRE) responsible for the induction of a wide range of protective enzymes involved in the synthesis of GSH and protection of cells against electrophilic as well as oxidative stress [15,20].Due to the reactive electrophilic activity of …
WebABGENEX develops and commercializes novel reagents for the scientific study of human biology and diseases and for the production of new diagnostic assays and potential therapies for such diseases. Areas of biological interest at IMGENEX India include cancer, apoptosis (programmed cell death), molecular signaling pathways, cellular aging, … WebThe ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, …
WebAug 6, 2013 · In the present study, Nrf2 activators were identified using cell-based luciferase enzyme fragment complementation (EFC) assay, and the mechanism of Nrf2 activation …
WebFor the NRF2 reporter gene experiments, the results were presented as the Induction Factor (IF), which is the measured relative light unit (RLU) value divided by the mean RLU value of the solvent control. When the induction factor of curcumin was over 8, the NRF2-U2OS reporter gene assay was regarded to be effective. copy and paste from imageWebAs useful tools for scientific research, reporter stable cell lines are those cells involved in expression of reporter protein (s) which are engineered through integrating the reporter expression cassettes into cell genomes mediated by plasmid or lentivirus vectors. In the studies of life science and biotechnology, the commonly used reporter ... copy and paste french lettersWeb双荧光素酶报告基因检测（Dual-Luciferase Reporter Assay）通常以萤火虫荧光素酶 (Firefly luciferase)为报告基因，以海肾萤光素酶 (Renilla luciferase)为内参基因。. 所构成的报告系统具有灵敏度高、动态范围广、应用灵活等优势，广泛用于基因调控、非编码RNA靶向互作等 ... famous people by centuryWebCell-Based Assays Luciferase Reporter Stable Cell... famous people buried in winchester cathedralWebThe ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, … famous people buried in yorkWebINDIGO’s reporter cells contain the receptor of interest and the luciferase reporter gene. Reporter cells have been optimized to provide extreme sensitivity to quantify even small … famous people buried near meWebGUS reporter system. The GUS reporter system ( GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique. copy and paste from excel